Plasmid

Part:BBa_K4739014:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-11)


Knockout plasmid for exoT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1060
    Illegal PstI site found at 1646
    Illegal PstI site found at 1771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1060
    Illegal NheI site found at 3719
    Illegal PstI site found at 1646
    Illegal PstI site found at 1771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1060
    Illegal BglII site found at 1755
    Illegal BglII site found at 4276
    Illegal XhoI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1060
    Illegal PstI site found at 1646
    Illegal PstI site found at 1771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7
    Illegal EcoRI site found at 1060
    Illegal PstI site found at 1646
    Illegal PstI site found at 1771
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 304
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 1197
    Illegal NgoMIV site found at 1445
    Illegal NgoMIV site found at 4612
    Illegal NgoMIV site found at 4622
    Illegal AgeI site found at 894
    Illegal AgeI site found at 996
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1469
    Illegal SapI.rc site found at 5890


Design Notes

The melting temperature and GC content of KO primer.


Source

The sequence of the homologous arm of exoS is from the genome of P. aeruginosa through Gibson assembly.

References